Oxidative stress, driven by reactive oxygen species (ROS) imbalance, causes cellular damage and chronic inflammation via positive feedback loop. Extracellular SOD3 convert superoxide to hydrogen peroxide, while GPX3 reduces it to water, providing synergistic neutralizing potential. We are developing a bicistronic co-expression construct using IRES2 and specific minding mRNA processing and protein secretion into extracellular space. The construct will be cloned into pcDNA3.4 under CMV promoter. Functionality of this construct will be assessed in HEK293T cells via assays suitable for mRNA and protein levels. This approach may enhance antioxidant defense in regenerative medicine and oxidative stress-related pathologies.

Бордина Ю.И. (науч. рук. Велегжанинов И.О.) Development and functional evaluation of a gpx3 and sod3 co-expression construct in eukaryotic cells // Сборник тезисов докладов конгресса молодых ученых. Электронное издание. – СПб: Университет ИТМО, [2026]. URL: https://kmu.itmo.ru/digests/article/16138