The relevance of this work stems from the need to develop sustainable methods for biofuel production, specifically isobutylene, using genetic engineering. The aim of this study is a comparative analysis of three distinct genetic constructs to optimize the expression of the α-ketoisocaproate dioxygenase (KICD) gene found in Rattus Norvegicus in Escherichia coli strain BL21(DE3). The research examines 3 vectors: the high-copy plasmid pUC19 with a lac promoter, the low-copy inducible plasmid pET-28(a)+ with a T7 promoter, and a modified pET-28(a)+ construct containing the synthetic constitutive promoter PJ23118. It is assumed that different vectors will have varying effects on the metabolic load of the cell and the expression level of the target gene as well as the produced protein function.

Федин Е.С., Заплавная С.С. (науч. рук. Велегжанинов И.О.) Comparative analysis of the influence of three different genetic constructs on kicd protein expression // Сборник тезисов докладов конгресса молодых ученых. Электронное издание. – СПб: Университет ИТМО, [2026]. URL: https://kmu.itmo.ru/digests/article/16060