This study aims to engineer Escherichia coli for cellulose-based isobutene production. A heterologous cellulase (Cel-Tfu) will be designed for extracellular cellulose hydrolysis, generating glucose as a carbon source. Through metabolic engineering, a codon-optimized α-ketoisocaproate dioxygenase (KICD) from Rattus norvegicus will convert α-ketoisocaproate into isobutene, which will be quantified using gas chromatography. This research establishes a synthetic pathway for sustainable isobutene production.

Заплавная С.С., Эльдиб А.А., Велегжанинов И.О. (науч. рук. Эльдиб А.А.) Engineering a synthetic metabolic pathway in escherichia coli for cellulose-dependent isobutene production // Сборник тезисов докладов конгресса молодых ученых. Электронное издание. – СПб: Университет ИТМО, [2025]. URL: https://kmu.itmo.ru/digests/article/14300