CRISPR/Cas9 has become very popular among scientists because of its simplicity and accessibility. Its greatest advantage is that it works on the principle through Waston-Crick base-pairing between sgRNA and target DNA. Unlike ZFN and TALEN, CRISPR does not require protein engineering, easy to construct and simple to use. Despite all the advantages of the CRISPR/Cas9 system, it cannot yet be used for gene editing because of its off-target effect[1]. This is why we are proposing a new nucleic acid-based gene therapy method for the double-stranded DNA cleavage. We hypothesize that its protein-free structure, design and Waston-Crick base pairing principle will allow it to achieve high specific double-stranded DNA cleavage and provide the lower cost of the suggested method.
Эльдиб А.А., Гайфуллина Л.М. (науч. рук. Колпащиков Д.М.) DEVELOPMENT OF DNA-MACHINE FOR DOUBLE STRANDED DNA CLEAVAGE // Сборник тезисов докладов конгресса молодых ученых. Электронное издание. – СПб: Университет ИТМО, [2023]. URL: https://kmu.itmo.ru/digests/article/10426