One of the promising methods of gene therapy is the technology of antisense oligonucleotides (ASO). However, they have the disadvantage of insufficient selectivity, which can lead to the death of healthy cells. In this work, using their modified version - binary ASOs, as well as additional sequences, we have achieved the creation of constructs that are able to recognize and respond only to high concentrations of the miRNA-17 oncomarker, as well as to distinguish it from the normal cell metabolite miRNA-20.
Курбанов Г.Ф., Мустафа А. (науч. рук. Колпащиков Д.М.) DEVELOPMENT OF A HIGHLY SELECTIVE BINARY ANTISENSE OLIGONUCLEOTIDE-BASED CONSTRUCT TO CLEAVE THE TARGET MRNA OF THE GFP GENE // Сборник тезисов докладов конгресса молодых ученых. Электронное издание. – СПб: Университет ИТМО, [2023]. URL: https://kmu.itmo.ru/digests/article/10147